WebMar 22, 2024 · Following the synthesis of parent dyes, one of their pendant alkyl chains was functionalized with a monomethoxytrityl protective group with the remaining hydroxyl … Web2.1 Choice of labeling sites. As the RNA is extended in the hybrid duplex, placement of a Cy3 dye toward the 5′ end of the RNA and the Cy5 dye at the 3′ end will result in a low …
Molecular structure of a Cy5... Download Scientific Diagram
WebCy5-alkyne. 95% (HPLC) ... Azide modified cyanine dye that can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition. ... Chemical Synthesis, Chromatography, Analytical and many others. Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … microsoft teams time tracker
Sequence-Dependent Fluorescence of Cyanine Dyes on Microarrays
WebMar 16, 2016 · To quantify sequence dependent fluorescence, we have measured the fluorescence intensity of Cy3 and Cy5 bound to the 5' end of all 1024 possible double-stranded DNA 5mers. The fluorescence intensity was also determined for these dyes bound to the 5' end of fixed-sequence double-stranded DNA with a variable sequence 3' … WebThe cyanine dyes Cy3 and Cy5 have proven valuable in numerous applications involving conjugation with proteins. Practical syntheses of lysine-selective, succinimidyl ester … microsoft teams time zone is wrong