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Cy5 dye synthesis

WebMar 22, 2024 · Following the synthesis of parent dyes, one of their pendant alkyl chains was functionalized with a monomethoxytrityl protective group with the remaining hydroxyl … Web2.1 Choice of labeling sites. As the RNA is extended in the hybrid duplex, placement of a Cy3 dye toward the 5′ end of the RNA and the Cy5 dye at the 3′ end will result in a low …

Molecular structure of a Cy5... Download Scientific Diagram

WebCy5-alkyne. 95% (HPLC) ... Azide modified cyanine dye that can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition. ... Chemical Synthesis, Chromatography, Analytical and many others. Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … microsoft teams time tracker https://epicadventuretravelandtours.com

Sequence-Dependent Fluorescence of Cyanine Dyes on Microarrays

WebMar 16, 2016 · To quantify sequence dependent fluorescence, we have measured the fluorescence intensity of Cy3 and Cy5 bound to the 5' end of all 1024 possible double-stranded DNA 5mers. The fluorescence intensity was also determined for these dyes bound to the 5' end of fixed-sequence double-stranded DNA with a variable sequence 3' … WebThe cyanine dyes Cy3 and Cy5 have proven valuable in numerous applications involving conjugation with proteins. Practical syntheses of lysine-selective, succinimidyl ester … microsoft teams time zone is wrong

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore …

Category:Novel asymmetric Cy5 dyes: Synthesis, photostabilities …

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Cy5 dye synthesis

Cy5 Fluorescent Dye Oligonucleotide Labeling - biosyn.com

WebJul 25, 2011 · Cy3 and Cy5 are among the most commonly used oligonucleotide labeling molecules. Studies of nucleic acid structure and dynamics use these dyes, and they are ubiquitous in microarray experiments. They are sensitive to their environment and have higher quantum yield when bound to DNA. The fluorescent intensity of terminal cyanine … WebThe fluorescent intensity of Cy3 and Cy5 dyes is strongly dependent on the nucleobase sequence of the labeled oligonucleotides. Sequence-dependent fluorescence may significantly influence the data obtained from many common experimental methods based on fluorescence detection of nucleic acids, such as sequencing, PCR, FRET, and FISH. …

Cy5 dye synthesis

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WebFigure 5. Gibbs free energy of solvation (ΔGsolv) calculated using Equation (1) for singly and doubly substituted Cy5 dyes in (a) water and (b) pyridine, quinoline, and isoquinoline. Singly and ... WebContact us if your fluorescent dye is not on the list. Featured: AZDye or Alexa Fluor 647 labeling, Cy3, Cy5, Cy7 Labeling (Structurally, AZDye 647 and Alexa Fluor 647 have the …

WebTo demonstrate the multiplexing capability of the iMS technique, a second iMS nanoprobe labeled with a different Raman dye, Cy5.5, was designed to target miR-34a. MiR-21 and … WebCyanine dyes do not localize to the nucleus. HeLa cells were treated with 10 µM Cy3, Cy5, Cy3-Cy5 or Cy3-Cy5-R8 in serum free media at 37 °C for 1 h before staining the nucleus with Hoechst 33258 and imaging. Excitation wavelength for Hoechst 33258 (nucleus stain), Cy3, and Cy5 is 405, 543, and 633 nm, respectively. Scale bars denote 25 µm.

WebFor the synthesis of the methyl Cy5 dye and its NHS ester (see Fig. 3 ), the three-carbon spacer (15) had to be prepared. Conden- sation of commercially available malondialdehyde bis (dimethyl ... WebWe have found that when single-stranded DNA is labeled with equivalent levels of Alexa Fluor 647 dye or Cy5 dye using aminoallyl dUTP incorporation, the fluorescence emission from the Alexa Fluor 647 dye–labeled DNA is several times more intense that that of Cy 5 dye–labeled DNA; similarly, Alexa Fluor 555 dye–labeled DNA is consistently ...

WebAug 31, 2024 · (A) Oligonucleotide sequence design for 3′-Cy3 and Cy5 labeled single-stranded DNA. The oligonucleotides consist of a 3′ terminal dye followed by the …

WebBio-Synthesis offers Cy5 fluorescent dye oligonucleotide labeling. Cy5 dye is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, … microsoft teams timezone is wrongWebSelect search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources microsoft teams timestamps wrongWebFor example, to label 3 mg of BSA (molar weight 66500 Dalton) with Cy5 NHS ester (molar weight 616 Dalton), and obtain maximum yield of mono-labeled product, one should use 8 × 3 mg × 616 Da / 66500 Da = 0.22 mg of Cy5 dye NHS ester. Calculate the volume of the reaction mixture. microsoft teams tipps tricks