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Gapdh antibody dilution

WebThere are 12 GAPDH antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA, IP, ICC/IF. There are 9 GAPDH antibody for WB, 4 GAPDH antibody for IHC-P, 3 GAPDH antibody for ELISA, 4 GAPDH antibody for IP, 2 GAPDH antibody for ICC/IF. Among all these GAPDH antibodies, there are 3 anti … WebMar 31, 2024 · The antibody dilutions were 800-fold in most cases except for GAPDH (5000-fold dilution), B-ACT and ALB (2000-fold dilution). Washing 5 times with T-TBS (200 µL/well). Incubation with secondary antibody 4000-fold diluted with blocker for 1 h at 37 °C with 400 rpm in the orbital shaker (100 µL/well).

GAPDH Monoclonal Antibody (6C5) (AM4300) - Thermo Fisher …

WebMonoclonal Anti-GAPDH antibody produced in mouse is suitable for western blotting using: protein extracted from heart tissue of mice at a working dilution of 1:25,000 3 myelin … Webfollowed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate. arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at ... brother printer ink lc71 lc75 black https://epicadventuretravelandtours.com

GAPDH Polyclonal Antibody (PA1-16777) - Thermo Fisher Scientific

WebIn Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ... Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements. WebJan 6, 2024 · GAPDH Antibody (H-12) is an IgG2a κ mouse monoclonal GAPDH antibody that detects m, r, and h GAPDH by WB, IP, IF, IHC(P) and ELISA. 245 citations ... Rated 5 out of 5 by Angelika from very good antibody works on WB in dilution 1:500 in 5%BSA on mouse brain tissue. Date published: 2024-02-15. Rated 3 out of ... http://biopioneer.com.tw/?p=41026 brother printer ink lc985

GAPDH Antibody (1D4) (NB300-221): Novus Biologicals

Category:Anti GAPDH (N-Terminal) Antibody Bio-Rad

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Gapdh antibody dilution

GAPDH monoclonal antibody Western, ELISA WH0002597M1

WebGlyceraldehyde-3-phosphate dehydrogenase (GAPDH), a classic glycolytic enzyme, is a multi-functional protein. It is mainly present in the cytoplasm. GAPDH is ubiquitously … WebWestern blot analysis of GAPDH in A) PC-12 whole cell lysate, B) rat2 whole cell lysate using GAPDH polyclonal antibody (Product # PA5-85074) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. ... Proteins were transferred to a membrane and probed with a …

Gapdh antibody dilution

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Web11 rows · Anti-GAPDH, Cat. No. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western … WebThe cells were labeled with GAPDH Mouse Monoclonal Antibody (Product # AM4300) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with …

WebSee all GAPDH antibodies. Applications Immunofluorescence analysis of GAPDH (green) showing staining in the cytoplasm of HeLa cells (right), compared with a negative control …

WebWestern blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody HRP-60004 at a dilution of 1:10000. WB analysis of HeLa using HRP-60004 HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. WebIncubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product webpage) in 10 ml primary antibody dilution buffer with …

WebDilution used 1:300 : Simple Western: Rat: Yang W, Han B, Chen Y, Geng F SAAL1, a novel oncogene, is associated with prognosis and immunotherapy in multiple types of cancer Aging 2024-08-13 [PMID: 35963646] (WB, Human) ... Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would …

WebThere are 12 GAPDH antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA, IP, ICC/IF. There are 9 GAPDH antibody for … brother printer ink mfc l8900cdwWebA specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing … brother printer ink levels checkWebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品 … brother printer ink nz